Creatine Phosphokinase Isoenzymes

Kinetic properties of the isoenzymes of human creatine phosphokinase.

Studies of the three human creatine phosphokinase (EC 2.7.3.2) isoenzymes, MM, MB, and BB, show that important differences exist in substrate dependency of the reaction rates. A method was developed to study these properties in which the ATP formed in the reverse reaction was measured by means of firefly luciferase. With substrate conditions at which the isoenzymes showed substantial difference...

Analysis of tissue creatine phosphokinase isoenzymes by histoelectrophoresis.

Cardiac Specific Creatine Phosphokinase

The specificity and sensitivity of serum creatine phosphokinase cardiac specific isoenzyme (MB) in the diagnosis of acute myocardial infarction (AM I) was evaluated. An ion-exchange chromatographic technique was used to isolate MB. Sera layered on mini-columns of DEAE-Sephadex were eluted with Tris-buffered sodium chloride. Quantification of isolated MB was performed by creatine phosphokinase (...

Lactate dehydrogenase and creatine phosphokinase isoenzymes in tissues of laboratory animals.

The lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) isoenzyme distributions in tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were analyzed by histoelectrophoresis. Tissues obtained were as follows: liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, kidney, spleen, lymph node, cerebrum, spinal cord and erythrocyte. Histoelectrophoresis wa...

Plasma Creatine Phosphokinase in Schizophrenia

Plasma creatine phosphokinase (GPK) was estimated from unmedicated schizophrenics to evaluate its significance in the diagnosis. The mean value of CPK in schizophrenics is 78.41±5.78 IU/L (n=49) as against control value of 27.45±2.27 IU/L (n = 36). The statistical analysis showed significance (p<0.05). The study finds its importance in view of the earlier reports suggesting the diagnostic value...